Preliminary crystallographic analysis of two hypothetical ribose-5-phosphate isomerases fromStreptococcus mutans
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چکیده
منابع مشابه
Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans.
Streptococcus mutans dextranase hydrolyzes the internal α-1,6-linkages of dextran and belongs to glycoside hydrolase family 66. An N- and C-terminal deletion mutant of S. mutans dextranase was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 1.6 Å and belonged to space group P2(1), with unit-cell parameters a = 53.2, b = 89.7, c = 63.3 Å, β = ...
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SAV0479, a hypothetical protein from the Mu50 strain of methicillin- and vancomycin-resistant Staphylococcus aureus, was selected for structure and function determination as part of a structural genomics project. Here, the cloning, overexpression, purification and crystallization of SAV0479 are reported. Crystals were obtained by the hanging-drop vapour-diffusion method and diffraction data wer...
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SA0961 is an unknown hypothetical protein from Staphylococcus aureus that can be identified in the Firmicutes division of Gram-positive bacteria. The gene for the homologue of SA0961 in Bacillus subtilis, ylaN, has been shown to be essential for cell survival, thus identifying the protein encoded by this gene as a potential target for the development of novel antibiotics. SA0961 was cloned and ...
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A patient with a lysosomal storage disease, progressive neurologic degeneration, and renal failure was found to have accumulated a low molecular weight nin-hydrin and phenol-HzS04 reactive compound. Amino acid analysis and gas chromatography-mass spectrometry identified a glutamic acid moiety. Direct insertion mass spectrometry proved the carbohydrate portion to be a sugar phosphate. NaB3H4 red...
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During a study of the energy-rich phosphorus compounds of higher plants, it became necessary to differentiate between ribose-3-phosphate and ribosed-phosphate. The only method available for distinguishing between these esters at low levels (quantities of 1 mg. or less) is that of Levene and Stiller (1). This method depends on the difference in the rate at which these compounds release their pho...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section F Structural Biology and Crystallization Communications
سال: 2012
ISSN: 1744-3091
DOI: 10.1107/s1744309112009761